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KMID : 1132720200180010004
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Genomics & Informatics
2020 Volume.18 No. 1 p.4 ~ p.4
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Quantitative evaluation of the molecular marker using droplet digital PCR
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Shin Won-Seok
Kim Han-Eul
Oh Dong-Yep
Kim Dong-Hee
Han Kyu-Dong
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Abstract
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Transposable elements (TEs) constitute approximately half of Bovine genome. They can be a powerful species-specific marker without regression mutations by the structure variation (SV) at the time of genomic evolution. In a previous study, we identified the Hanwoo-specific SV that was generated by a TE-association deletion event using traditional PCR method and Sanger sequencing validation. It could be used as a molecular marker to distinguish different cattle breeds (i.e., Hanwoo vs. Holstein). However, PCR is defective with various final copy quantifications from every sample. Thus, we applied to the droplet digital PCR (ddPCR) platform for accurate quantitative detection of the Hanwoo-specific SV. Although samples have low allele frequency variation within Hanwoo population, ddPCR could perform high sensitive detection with absolute quantification. We aimed to use ddPCR for more accurate quantification than PCR. We suggest that the ddPCR platform is applicable for the quantitative evaluation of molecular markers.
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KEYWORD
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droplet digital PCR, Hanwoo-specific marker, structure variation
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